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Proteins from whole cell extracts (WCE) were obtained by resuspending cells in conventional Triton X-100 lysis buffer complemented with protease inhibitors (50 mM Tris, pH 7.4; 150 mM NaCl; 50 mM NaF; 5 mM EDTA; 10% glycerol; 1 mM Na 3 VO 4; 40 mM β-glycerophosphate; 0.1 mM phenylmethylsulfonyl fluoride; 5 μg/ml … Add To Cart. Choose bestseller bundles, variety packs or shop for individual bars.